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Preclinical in vitro dose-response analysis of CSEO and CHX against Porphyromonas gingivalis and <t>in</t> <t>BALB/3T3</t> fibroblasts. Before the clinical trial, in vitro assays were performed to identify concentrations with antimicrobial activity and to assess mammalian cell cytotoxicity. ( A ) Growth of P. gingivalis after exposure to CSEO or CHX, measured by optical density (OD) and normalized to the maximum background-corrected mean OD observed in the assay. Lower values indicate stronger growth inhibition. ( B ) BALB/3T3 fibroblast viable cell count after 24 h exposure to CSEO or CHX. CSEO values were normalized to the DMSO vehicle control, whereas CHX values were normalized to untreated cultures. For both curves, the corresponding control mean was defined as 100%. The horizontal line marks 50% of the corresponding control. Points with error bars represent experimental measurements, and solid lines represent fitted dose-response curves. ( C ) Model-derived IC50 estimates with 95% profile-likelihood confidence intervals. *For the antibacterial assay, IC50 values are reported as relative IC50 estimates, defined as the concentration producing a fitted response halfway between the fitted top and bottom plateaus
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Preclinical in vitro dose-response analysis of CSEO and CHX against Porphyromonas gingivalis and <t>in</t> <t>BALB/3T3</t> fibroblasts. Before the clinical trial, in vitro assays were performed to identify concentrations with antimicrobial activity and to assess mammalian cell cytotoxicity. ( A ) Growth of P. gingivalis after exposure to CSEO or CHX, measured by optical density (OD) and normalized to the maximum background-corrected mean OD observed in the assay. Lower values indicate stronger growth inhibition. ( B ) BALB/3T3 fibroblast viable cell count after 24 h exposure to CSEO or CHX. CSEO values were normalized to the DMSO vehicle control, whereas CHX values were normalized to untreated cultures. For both curves, the corresponding control mean was defined as 100%. The horizontal line marks 50% of the corresponding control. Points with error bars represent experimental measurements, and solid lines represent fitted dose-response curves. ( C ) Model-derived IC50 estimates with 95% profile-likelihood confidence intervals. *For the antibacterial assay, IC50 values are reported as relative IC50 estimates, defined as the concentration producing a fitted response halfway between the fitted top and bottom plateaus
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Preclinical in vitro dose-response analysis of CSEO and CHX against Porphyromonas gingivalis and <t>in</t> <t>BALB/3T3</t> fibroblasts. Before the clinical trial, in vitro assays were performed to identify concentrations with antimicrobial activity and to assess mammalian cell cytotoxicity. ( A ) Growth of P. gingivalis after exposure to CSEO or CHX, measured by optical density (OD) and normalized to the maximum background-corrected mean OD observed in the assay. Lower values indicate stronger growth inhibition. ( B ) BALB/3T3 fibroblast viable cell count after 24 h exposure to CSEO or CHX. CSEO values were normalized to the DMSO vehicle control, whereas CHX values were normalized to untreated cultures. For both curves, the corresponding control mean was defined as 100%. The horizontal line marks 50% of the corresponding control. Points with error bars represent experimental measurements, and solid lines represent fitted dose-response curves. ( C ) Model-derived IC50 estimates with 95% profile-likelihood confidence intervals. *For the antibacterial assay, IC50 values are reported as relative IC50 estimates, defined as the concentration producing a fitted response halfway between the fitted top and bottom plateaus
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human foreskin fibroblast cell line  (ATCC)
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Cytotoxic effects of the selected compounds on HFF‐1 cells.
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Cytotoxic effects of the selected compounds on HFF‐1 cells.
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Preclinical in vitro dose-response analysis of CSEO and CHX against Porphyromonas gingivalis and in BALB/3T3 fibroblasts. Before the clinical trial, in vitro assays were performed to identify concentrations with antimicrobial activity and to assess mammalian cell cytotoxicity. ( A ) Growth of P. gingivalis after exposure to CSEO or CHX, measured by optical density (OD) and normalized to the maximum background-corrected mean OD observed in the assay. Lower values indicate stronger growth inhibition. ( B ) BALB/3T3 fibroblast viable cell count after 24 h exposure to CSEO or CHX. CSEO values were normalized to the DMSO vehicle control, whereas CHX values were normalized to untreated cultures. For both curves, the corresponding control mean was defined as 100%. The horizontal line marks 50% of the corresponding control. Points with error bars represent experimental measurements, and solid lines represent fitted dose-response curves. ( C ) Model-derived IC50 estimates with 95% profile-likelihood confidence intervals. *For the antibacterial assay, IC50 values are reported as relative IC50 estimates, defined as the concentration producing a fitted response halfway between the fitted top and bottom plateaus

Journal: Clinical Oral Investigations

Article Title: Salivary microbiome responses to chlorhexidine and Coriandrum sativum essential oil mouthwashes in patients with periodontitis in a randomized 2-week clinical trial

doi: 10.1007/s00784-026-07198-8

Figure Lengend Snippet: Preclinical in vitro dose-response analysis of CSEO and CHX against Porphyromonas gingivalis and in BALB/3T3 fibroblasts. Before the clinical trial, in vitro assays were performed to identify concentrations with antimicrobial activity and to assess mammalian cell cytotoxicity. ( A ) Growth of P. gingivalis after exposure to CSEO or CHX, measured by optical density (OD) and normalized to the maximum background-corrected mean OD observed in the assay. Lower values indicate stronger growth inhibition. ( B ) BALB/3T3 fibroblast viable cell count after 24 h exposure to CSEO or CHX. CSEO values were normalized to the DMSO vehicle control, whereas CHX values were normalized to untreated cultures. For both curves, the corresponding control mean was defined as 100%. The horizontal line marks 50% of the corresponding control. Points with error bars represent experimental measurements, and solid lines represent fitted dose-response curves. ( C ) Model-derived IC50 estimates with 95% profile-likelihood confidence intervals. *For the antibacterial assay, IC50 values are reported as relative IC50 estimates, defined as the concentration producing a fitted response halfway between the fitted top and bottom plateaus

Article Snippet: To assess mammalian cell cytotoxicity, BALB/3T3 clone A31 mouse fibroblasts (ATCC CCL-163; American Type Culture Collection, Manassas, VA, USA, RRID:CVCL_0184) were used at post-thaw passages 3–18.

Techniques: In Vitro, Analysis, Activity Assay, Inhibition, Cell Characterization, Control, Derivative Assay, Concentration Assay

Journal: Archiv Der Pharmazie

Article Title: Spirooxindole 5‐(Trifluoromethyl)benzothiazole Hybrids: Synthesis, Crystal Structure, and Antioxidant Activity

doi: 10.1002/ardp.70343

Figure Lengend Snippet: Cytotoxic effects of the selected compounds on HFF‐1 cells.

Article Snippet: Human foreskin fibroblast cell line (HFF‐1, ATCC, SCRC‐1041) was used in the study.

Techniques: